Product Description. ClonExpress MultiS One Step Cloning Kit (Vazyme #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit up to 5 fragments can be orderly assembled in a single
2021-6-18 · ClonExpress MultiS One Step Cloning Kit C113 ClonExpress® MultiS One Step Cloning Kit
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
ClonExpress UItra One Step Cloning kit (Vazyme #C115) is a novel cloning kit applicable for homologous recombination with 15 fragments. The Kit is independent of DNA ligase significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized buffer included in 2x ClonExpress Mix significantly improve the
2021-3-15 · VazymeBiotechCo. Ltd Order global vazyme m Support global vazyme m ww w.vazyme m Forresearchuseon ly notforuseindiagnosticprocedures. M DL5 000Marker.
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul 37℃ 30min
Molecular Cloning/Mutagenesis-Vazyme Biotech Co. Ltd. ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector.
2021-2-4 · The construction of the plasmids was achieved by homologous recombination with the Exnase enzyme (Vazyme C113). Yeast transformation was conducted according to the manual (PT1172-1) from Clontech Laboratories
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul 37℃ 30min
2021-6-30 · DNA fragments encoding human ubiquitin-specific peptidase 10 (USP10) double-stranded RNA-dependent protein kinase (PKR) and G3BP2 were PCR-amplified from cDNA that was reverse transcribed from HEK293T total RNA with reverse transcriptase (Vazyme Nanjing China) according to the manufacturer s instructions.
Rapid Cloning -Vazyme Biotech Co. Ltd. ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone
2020-6-23 · Abstract Cytophaga hutchinsonii is an aerobic cellulolytic soil bacterium that rapidly digests crystalline cellulose. The predicted mechanism by which C. hutchinsonii digests cellulose differs from that of other known cellulolytic bacteria and fungi. The genome of C. hutchinsonii contains 22 glycoside hydrolase (GH) genes which may be involved in cellulose degradation. One predicted GH with
2021-6-18 · ClonExpress MultiS One Step Cloning Kit C113 ClonExpress® MultiS One Step Cloning Kit
2020-9-7 · resultant PCR products were ligated by Exnase II (Vazyme Co. Ltd) into pET28a double-digested with BamH I and Xho I and transformed into E. coli BL21(DE3). Positive transformants evaluated by colony PCR and Fig. 1. Screening results of CHCM hydrolase-producing strains.
2019-3-23 · As for the ligation process we use the T4 ligase from Thermo Scientific (#EL0011) or Exnase from Vazyme (C112-01/02). Ligation using T4 ligase. All of the ligation process we did using T4 ligase were between sticky ends the reaction mixture was as following
2015-9-15 · In this study we developed a rapid and efficient Exnase TM II-based in vitro recombination approach for influenza gene cloning into reverse genetics system. And the high efficacy of the strategy was evaluated and confirmed by cloning the eight genes from viruses PR8 and H9N2 into the reverse genetics system. 2.
2021-2-2 · recombination with the Exnase enzyme (Vazyme C113). Yeast transformation was conducted according to the manual (PT1172-1) from Clontech Laboratories and two-hybrid assays were performed as we previously described.33 We plated the mating products on SD/ Trp/ Leu ( LW) and SD/ Trp/ Leu/ His/ Ade ( LWHA)platesfor3–5daystotesttheinter-actions.
2019-3-21 · VazymeBiotechCo. Ltd Order global vazyme Support global vazyme vazyme Forresearchuseonly notforuseindiagnosticprocedures. M DL5 000Marker.
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2021-7-20 · ClonExpress II One Step Cloning Kit C112 ClonExpress® II One Step Cloning Kit
ClonExpress® DNA . ClonExpress®II Exnase®II . / PCR 5 PCR 5 3 (15 bp-20 bp) .
2019-3-21 · VazymeBiotechCo. Ltd. Order global vazyme Support support vazyme vazyme Forresearchuseonly notforuseindiagnosticprocedures. Inserts can be amplifiedby any polymerase (i.e. Taq DNA polymerase or high-fidelitypolymerase). It will not interfere with the recombina-
Rapid Cloning -Vazyme Biotech Co. Ltd. ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone
2021-2-4 · The construction of the plasmids was achieved by homologous recombination with the Exnase enzyme (Vazyme C113). Yeast transformation was conducted according to the manual (PT1172-1) from Clontech Laboratories
2019-11-1 · The tcpA constructs were obtained from SC09 genomic DNA by recombinase Exnase II (ClonExpress II Vazyme) and then cloned in pCMV-Tag 2B. 2.9. Co-immunoprecipitations (CO-IP) Rainbow trout head kidney macrophages were infected with the wild-type SC09 for 12 h. Cells were washed twice in ice-cold PBS harvested and cell lysis buffer was added.
The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification greatly simplifying The experimental steps.
2013-10-31 · ClonExpress MultiS.. Vazyme biotech co. ltd. vazyme /Web vazyme /Tel 400-600-9335 /Sales sales vazyme /Support support vazyme /Service service vazyme Vazyme Biotech Co. Ltd
2020-1-31 · .pdf ® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co. ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03
C115-01/02. ClonExpress®UltraOneStepCloningKit 5-15min Mix 50bp 10kb >95 / PCR 5 3 . 800.00.
2019-3-23 · As for the ligation process we use the T4 ligase from Thermo Scientific (#EL0011) or Exnase from Vazyme (C112-01/02). Ligation using T4 ligase. All of the ligation process we did using T4 ligase were between sticky ends the reaction mixture was as following
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2021-3-15 · VazymeBiotechCo. Ltd Order global vazyme m Support global vazyme m ww w.vazyme m Forresearchuseon ly notforuseindiagnosticprocedures. M DL5 000Marker.
Molecular Cloning/Mutagenesis-Vazyme Biotech Co. Ltd. ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector.
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2021-7-20 · ClonExpress II One Step Cloning Kit C112 ClonExpress® II One Step Cloning Kit
2019-11-1 · The tcpA constructs were obtained from SC09 genomic DNA by recombinase Exnase II (ClonExpress II Vazyme) and then cloned in pCMV-Tag 2B. 2.9. Co-immunoprecipitations (CO-IP) Rainbow trout head kidney macrophages were infected with the wild-type SC09 for 12 h. Cells were washed twice in ice-cold PBS harvested and cell lysis buffer was added.
2014-4-27 · ClonExpress. Vazyme biotech co. ltd. vazyme /Web vazyme /Tel 400-600-9335 /Sales sales vazyme /Support support vazyme /Service service vazyme Vazyme Biotech Co. Ltd Version 3.2 ClonExpress TM II
2019-1-31 · ClonExpress.pdf ClonExpressTM II One Step Cloning Kit Vazyme Biotech Co. Ltd w w /Web w . v /Tel 400-600-9335 a Vazyme biotech co. ltd. z y /Sales sales m e . c
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul 37℃ 30min
ClonExpress II One Step Cloning (Vazyme #C112) is a simple fast and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly the vector is
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